Imagine a spoon placed in half a glass of water. This is because the light rays reaching your eyes from under the water change direction when they pass into the air. Reflection with normal incidence means that the sound is traveling straight toward the tissue at a right angle. Reflection and Transmission Typical reflection and transmission curves for external reflection.These curves are the graphical representation of the Fresnel equations.Note that the reflected amplitude for the light polarized parallel to the incident plane is zero for a specific angle called the Brewster angle. Click to toggle author information(expand message area). The amplitude of the reflected light is equal to the amplitude of the incident light times the reflection coefficient of the surface. Use this technique on brightfield images. Luminous Intensity. You can correct uneven illumination or dirt/dust on lenses by acquiring a "flat-field" reference image with the same intensity illumination as the experiment.The flat field image should be as close as possible to a field of view of the cover slip without any cells/debris. When a beam of light illuminates a lipid vesicle, the light reflected from the vesicular concave membrane can be focused to have higher intensity and generate enhanced effects. Most of the things we see are because light from a source has reflected off it. The spoon appears to bend at the air-water boundary. You can find tables of refection coefficients for many different surfaces. You need to also check the subject to background distance. Reflection with normal incidence means that the sound is traveling straight toward the tissue at a right angle. When a beam of light illuminates a lipid vesicle, the light reflected from the vesicular concave membrane can be focused to have higher intensity and generate enhanced effects. The light would have to be psychic to adjust its intensity before it is incident into an interface. You can find tables of refection coefficients for many different surfaces. Flat-field correction Proper correction. Diffuse reflection is when light hits an object and reflects in lots of different directions. These are the two main aspects that govern the intensity of the light.. This means two different tissue interfaces. This means two different tissue interfaces. This phenomenon is known as refraction. Intensity of Light Intensity of the light in comparison to the subject can be controlled by changing the distance between the subject and the light. Luminous intensity is the luminous flux per unit solid angle, measured in candelas (cd).7,16,31,36 Luminance is a measure of brightness, that is, the amount of light per unit area, either emitted by or reflected from a surface, measured in candelas per square meter (cd/m2) or nits (nt, not officially recognized unit).